Table 4. Effects of various targeted drugs on survival (apoptosis) of myeloma cell lines.
| Drug | EC50 (μM) values produced by various drugs in | ||||
|---|---|---|---|---|---|
| MM.1S | NCI-H929 | OPM-2 | RPMI-8226 | U-266 | |
| BI2536 | 0.001-0.01 | 0.01 | 0.001-0.01 | 0.001-0.01 | 0.001-0.01 |
| Obatoclax | 0.5 | 0.5 | 0.001-0.01 | 0.01-0.1 | 0.5 |
| BEZ235 | 0.1-0.5 | 0.5 | 0.01-0.1 | 0.5 | 0.1-0.5 |
| 17AAG | 0.01-0.1 | 0.1-0.5 | 0.1-0.5 | 0.5 | 1 |
| VX-680 | 5 | 1-5 | 0.5-1 | 0.1-0.5 | 0.5 |
| Vorinostat | 1-5 | 1-5 | 1 | 1-5 | 0.5-1 |
| Nilotinib | 1-5 | 1-5 | 1-5 | 1-5 | 1-5 |
| Imatinib | >10 | >10 | >10 | >10 | >10 |
| Dasatinib | >10 | >10 | 5-10 | >10 | >10 |
| Bosutinib | 1-5 | 5 | 1-5 | 1-5 | 5 |
| Sorafenib | 5 | 1-5 | 1-5 | 5 | 1-5 |
| Sunitinib | 1 | 1-5 | 0.5-1 | 0.5 | 1 |
| Erlotinib | >10 | >10 | >10 | >10 | >10 |
| Gefitinib | >10 | >10 | >10 | >10 | >10 |
| Lapatinib | 5-10 | >10 | 5-10 | 5-10 | 5-10 |
Myeloma cell lines were incubated with increasing concentrations of various drugs (0.001-10 μM) at 37°C for 48 hours. Apoptosis was determined by active caspase-3 staining and flow cytometry as described in the text. Abbreviations: EC50, effective concentration (50%).