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. 2016 Aug 25;7(40):65660–65668. doi: 10.18632/oncotarget.11597

Figure 6. Genetic inhibition of autophagic flux promotes TRAIL-induced cell death upon EGCG treatment.

Figure 6

HCT116 cells were pre-treated with 20 nM ATG5 siRNA for 4 h, exposed to 20 μM EGCG for 12 h, and treated with 100 ng/ml TRAIL for 2 h. A. Cell morphology was photographed under a light microscope (×200); B, C. Viability of treated cells was measured by crystal violet staining. Viability of control cells was considered as 100%; D. LDH released into the cell culture medium was measured after exposure to TRAIL for 2 h; E. Western blot analysis of caspase-8, ATG5 and LC3. β-actin was used as loading control; **p < 0.01 indicating significant differences between control group and treatment group.