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. 2016 Aug 25;7(40):65707–65720. doi: 10.18632/oncotarget.11604

Figure 5. Effect of eupafolin plus TRAIL treatment on apoptosis of other carcinoma cells and normal cells.

Figure 5

A. U251MG (glioma cells) and DU145 cells (prostate cancer cells) were treated with 50 ng/ml TRAIL in the presence or absence of 30 μM eupafolin for 24 h. The level of apoptosis was assessed by measuring the sub-G1 fraction using flow cytometry (upper panel). The protein levels of PARP, Mcl-1, Bim, and actin were determined by western blotting. The level of actin was used as the loading control (lower panel). B. Caki, mesangial cells (MC) and mouse renal tubular epithelial (TCMK-1) cells were treated with50 ng/ml TRAIL in the presence or absence of 30 μM eupaforin for 24 h. The cell morphology was examined using interference light microscopy. The level of apoptosis was assessed by measuring the sub-G1 fraction using flow cytometry. C. MC and TCMK1 cells were treated with the indicated concentrations of eupafolin for 24 h. The protein levels of Mcl-1, Bim and actin were determined by western blotting. The level of actin was used as the loading control.