Figure 1. IL-32α expression is higher in migratory human melanoma cell lines.

A. The migratory abilities of human melanoma cell lines G361, A375, SK-MEL-5, SK-MEL-28, Hs 294T, and WM-266-4 were examined. Cells (5×10⁴) were placed in the transwell migration assay upper chambers and DMEM containing 5% FBS was placed in the lower chambers. After incubation for 24 hours, membranes with migrated cells were fixed and stained as described in Materials and Methods. Stained cells were evaluated by microscopy and photographed (magnification, 40×). Membranes were eluted as described and the O.D. at 570 nm was measured. B. The human melanoma cell lines were examined for IL-32α expression levels. The lanes of melanoma cell lines are organized in order of migratory ability. Western blotting was performed using the mouse anti-human IL-32α antibody.