Figure 2. IL-32α-overexpressing G361 cells exhibit a round phenotype.
A. G361 cells were transfected with pcDNA3.1+ or IL-32α/pcDNA3.1+ as described. Total RNA was isolated from transfectants (G361-vector and G361-IL-32α), and the expression level of IL-32α was assessed by RT-PCR. B. Transfectants (G361-vector and G361-IL-32α) were lysed and immunoblotted using the mouse anti-human IL-32α antibody to confirm IL-32α overexpression. C. G361, G361-vector, and G361-IL-32α cells (5×105) were seeded on culture plates and incubated for 24 hours. Cell morphology was evaluated by microscopy and photographed. Original magnification, 200×. Images are representative of three independent experiments.