Skip to main content
. 2016 Aug 30;7(40):65923–65936. doi: 10.18632/oncotarget.11688

Figure 3. Interaction of human Ikaros and FOXP1 in B-ALL cells and effect of the IK6 deletion.

Figure 3

A. A FLAG-tagged human FOXP1 construct was co-transfected into 293T cells with wild-type (wt) human Ikaros constructs (lanes 1 - 3 and 7 - 9), or human IK6 (lanes 4 - 6 and 10 - 12). Following IP with anti-FLAG or anti-Ikaros, samples were immunoblotted with anti-Ikaros (left panel) or anti-FLAG (FOXP1) (right panel). B. GST pulldown assay using a human GST-FOXP1 fusion and 35S labelled wt human Ikaros or the human IK6 isoform. C. Anti-Foxp1 IP of Ikaros isoforms in human leukemia cell lines. The REH line (left panel) expresses WT Ikaros. The SUP-B15 line (right panel) expresses wt Ikaros and the IK6 deletion isoform. Arrows indicate wt Ikaros, IK6, FLAG-Foxp1 and IgH on the gels.