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. 2016 Sep 1;7(40):66032–66050. doi: 10.18632/oncotarget.11797

Figure 2. Rig-G inhibits lung cancer cell growth.

Figure 2

A. The lung cancer cell (A549, H1792, and Calu-1) sublines pTRE and Tet-on Rig-G were cultured, respectively, in the presence or absence of Dox (2μg/mL) for 24h. The expression of Rig-G protein was detected by immunoblotting. B. The proliferation of the indicated cells was measured by ELISA (BrdU labeling) analysis. The results are expressed as the mean ± SEM, ***p < 0.001. C. The growth of the indicated cells in soft agar was assessed by using an anchorage-independent colony formation assay. Scale bars = 500 μm. D. The maximum colony size of A549, H1792, and Calu-1 cells in a soft-agar assay was determined. The results are expressed as the mean ± SEM, ***p < 0.001.