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. 2016 Sep 1;7(40):66032–66050. doi: 10.18632/oncotarget.11797

Figure 7. Effects of Rig-G protein on IκBα degradation.

Figure 7

A. Western blotting analysis of IκBα expression in A549 sublines pTRE and Tet-On Rig-G. The cells were cultured respectively in the presence or absence of Dox (2 μg/mL) for 24 h. B. The pTRE and Tet-On Rig-G cells were incubated with the proteasome inhibitor MG132 (20μM) for 5h, lysed, and subjected to immunoprecipitation (IP) with anti- IκBα antibody. The resulting precipitates were detected by Western blot analysis with antiubiquitin antibodies. C. NF-κB activation in the indicated cells untreated or treated with 1 ng/mL TNFα for 1 h. The results are expressed as the mean ± SEM (n = 3), ***p < 0.001. D. The effect of Rig-G on the TNF-mediated inducibility of an NF-κB reporter construct. E. The indicated cells were incubated with the proteasome inhibitor MG132 (20μM) for 5h, and cells were untreated or treated with 1 ng/mL TNFα for 1 h, and cell lysates were immunoprecipitated (IP) with an anti-IκBα antibody, followed by Western blot analysis with an anti-ubiquitin antibody.