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. 2016 Sep 1;7(40):66032–66050. doi: 10.18632/oncotarget.11797

Figure 8. NF-κB is important in RIG-G-induced cell growth inhibition.

Figure 8

A. A549 sublines pTRE and Tet-On Rig-G were transduced with indicated shRNA plasmids. After selection, the cells were cultured, respectively, in the absence or presence of Dox (2 μg/mL) for 24 h, expression of the indicated proteins was analysed. B. The indicated cells were transduced with control or p65 shRNA plasmids, and their proliferation was measured by ELISA (BrdU labeling) analysis. The results are expressed as the mean ± SEM, ***p < 0.001. C. The growth of tumor cells transduced with indicated shRNA plasmids in soft agar was assessed by using an anchorage-independent colony formation assay. Scale bars = 500 μm. D. The maximum colony size assay in A549 sublines pTRE and Tet-On Rig-G cells transfected with the indicated shRNA plasmids. The results are expressed as the mean ± SEM, **p < 0.01; ***p < 0.001.