Figure 2. MLN4924 causes G2/M cell cycle arrest in osteosarcoma cells.

Three osteosarcoma cell lines: MG63 A., Saos-2 B. and U2OS C. were treated with DMSO or MLN4924 (1 μM) for 24 and 48 h. Cells were harvested and fixed in ice-cold 70% ethanol overnight at −20°C, and then stained with PI (5 μg/100 μL) for 30 min at 4 °C in the dark. DNA profiles were analyzed by flow cytometry. > 4N cells were shown in D-F. Each value was the mean ± SEM of three replicates from a single assay.