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. 2016 Sep 1;7(40):66087–66099. doi: 10.18632/oncotarget.11807

Figure 3. MLN4924 induces apoptosis in MG63 and Saos-2, but not U2OS cells.

Figure 3

A-C. Three osteosarcoma cell lines MG63 (A), Saos-2 (B) and U2OS (C) were treated with DMSO or MLN4924 (1 μM) for 48 h. Cells were harvested and stained with Annexin V-FITC and PI for 20 min in the dark. Apoptosis was analyzed by flow cytometry. D. The graph illustrates the percentage of total apoptosis cells. Each value was the mean ± SEM of three replicates from a single assay. Q1: live cells (annexin V−/PI−), Q2: early apoptotic cells (annexin V+/PI−), Q3: late apoptotic cells (annexin V+/PI+) and Q4:necrotic cells (annexin V−/PI+).*P < 0.05, **P < 0.01 unpaired t test.