Table 2. PCR primers used in this study.
SNP_ID | 1st-PCR primer | 2nd-PCR primer | UEP_SEQ |
---|---|---|---|
rs6436917 | ACGTTGGATGGCCAATTGTAAGTGCCAAAG | ACGTTGGATGATGGCCCTAAAATGTTCCAC | ggAATGTTCCACAGTGGGC |
rs4327230 | ACGTTGGATGACAATAGCAAAGACATGGGC | ACGTTGGATGTATGGCTGCATAGTATTCCG | ACATATTCTTTATCCAGTCTATAATT |
rs2114591 | ACGTTGGATGGAGACGTACAGAAAAGAGGG | ACGTTGGATGCTTCCAACAACCACTGTCAC | aacaACTGTCACATCAACAGATCC |
rs7580912 | ACGTTGGATGACACCCTCTCTCCTAACTAC | ACGTTGGATGGTCTCAAACCACAAACCACC | aaaaaCACAAACCACCAGGGCTTCC |
rs13422 | ACGTTGGATGTTGGGATTTTGGGCTAGCTC | ACGTTGGATGTCTGAGCGTACATAGGGAAG | ggagcGGGAAGGGAGGAAGGGAAA |
rs230915 | ACGTTGGATGCAAGAAGCCTTTCAGTTGAG | ACGTTGGATGCCTGACTCTTAGTAAGTCAA | ttttcAGTAAGTCAATAGAGTGCTGC |
rs231024 | ACGTTGGATGAGCTGGATAAACAGGTCTGG | ACGTTGGATGCCATGCCTGGCCATCTTATT | gGGCCATCTTATTATTATTTAATGA |
UEP: Unextended mini-sequencing primer.