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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Nov 10;1861(2):246–255. doi: 10.1016/j.bbagen.2016.11.002

FIGURE 3. Processing and subcellular localization of ectopically expressed ΔSP- and FL-PDYN in RINm-5F cells.

FIGURE 3

A. ΔSP- and FL-PDYN proteins analyzed by western blotting with anti-PDYN CTF antibody in RINm-5F transfected cells.

B, C. The levels of prodynorphin derived dynorphin A (Dyn A), dynorphin B (Dyn B) and Leu-enkephalin-Arg (LER) in the RINm-5F transfected cells (B) and in the culture media (C) analyzed by RIA. The cells were transfected with pCMV vector (mock), pCMV-ΔSP-PDYN or pCMV-FL-PDYN. Data were normalized to the levels in mock transfected cells and are shown as mean ± SEM; n = 3 experiments.

D. Subcellular localization of ΔSP-PDYN protein in RINm-5F cells observed under confocal fluorescence microscopy. Transfected cultures were immunolabeled with antibodies against PDYN CTF or dynorphin A. Images were taken with × 100 objective and × 5 electron zoom factor. Scale bar, 30 µm.