TABLE 1.
Demographic data and tissue characteristics of human subjects
| Subject | Age (years) |
sex | PMI (hours) |
Brain pH | RQI | Brain structure | Assay | Smoking history |
Cause of death | Toxicological findings at time of death |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 (#71) | 23 | M | 41.7 | 6.96 | 8.1 | NAc, Cd, Put, OFC, PFC, Cbl |
qRT-PCR | Yes | muliple injuries, suicide |
None |
| 2 (#79) | 23 | M | 39.0 | 6.83 | 8.0 | NAc, Cd, Put, OFC, PFC, Cbl |
qRT-PCR | No | heart disease | None |
| 3 (#81) | 53 | F | 38.7 | 6.75 | 7.7 | NAc, Cd, Put, OFC, PFC, Cbl |
qRT-PCR | NA | multiple injuries, accidental death |
Ethanol, caffeine, zopiclone, oxazepam |
| 4 (#84) | 69 | M | 26.5 | 6.69 | 7.8 | NAc, Cd, Put, OFC, PFC, Cbl |
qRT-PCR | No | hanging, suicide | Oxazepam |
| 5 (#89) | 54 | M | 61.0 | 6.28 | 7.1 | NAc, Cd, Put, OFC, PFC, Cbl |
qRT-PCR | No | intoxalc, accidental death |
Ethanol, mirtazapine, promethazine, lactate, glucose |
| 6 (#80) | 59 | F | 47.3 | 6.27 | 6.8 | NAc, Cd, Put, OFC, PFC, Cbl |
qRT-PCR | Yes | perf duodenal ulcer | Ethanol, citalopram, desmethylcitalopram |
| 7 (#102) | 31 | M | 61.7 | 6.40 | 7.9 | NAc, Cd, Put, OFC, PFC, Cbl |
qRT-PCR | No | Rupture of the inferior vena, fracture liver damage |
Ketamine |
| 8 (#11) | 57 | M | 46.0 | NA | - | Str, Cbl | FANS, WB, RIA | NA | hypertensive cardiomyopathy |
NA |
| 9 (#184) | 21 | M | 44.0 | NA | - | Str, Cbl | FANS, WB, RIA | NA | hanging, suicide | Methylphenidate |
| 10 (#191) | 92 | M | 27.5 | 6.68 | - | Str, Cbl | FANS, WB, RIA | NA | hanging, suicide | NA |
| 11 (SW1) | 23 | F | 11.0 | 6.85 | - | Cd | IF | Yes | motor vehicle accident | None |
| 12 (SW2) | 46 | F | 24.0 | 6.32 | - | Cd | IF | No | homicide | None |
| 13 (SW3) | 42 | M | 20.0 | 6.82 | - | Cd | IF | NA | cardiac related | None |
| 14 (SW4) | 57 | M | 10.0 | 6.65 | - | Cd | IF | NA | cardiac related | None |
NAc, nucleus accumbens; Cd, caudate nucleus; Put, putamen; Str, striatum; OFC, orbitofrontal cortex; PFC, dorsolateral prefrontal cortex; Cbl, cerebellum.
qRT-PCR, quantitative real-time PCR; IF, immunofluorescence; FANS, fluorescent-activated nuclei sorting; WB, western blotting; RIA, radioimmunoassay; RQI, RNA quality indicator.
Toxicological parameters measured: ethanol, methanol, acetone, cannabis, amphetamines and opiates, cocaine metabolite, MDMA analogues, buprenorphine concentrations; drug screening.