Table 3.

B. subtilis strains used in this study.

Strain	Relevant genotype (reference)
AG174	trpC2 pheA1 (wild type, JH642) (Perego et al., 1988)
AIG200	trp+, Δ{oriC dnaA dnaN}::spc, spoIIIJ::{oriN repN kan}, amyE::(PxylA-dnaN cat) (Goranov et al., 2005)
CAL2074	Δ{oriC dnaA}::{oriN repN Ppen-2028-dnaN cat}
CAL2083	Δ{oriC dnaA}::{oriN repN Ppen-2028-dnaN cat}, lacA::{Pspank-dnaA tet}
CAS192	Δrok::cat::mls, Δ{oriC dnaA}::{oriN repN Ppen-2028-dnaN cat}
CAS196	Δrok::cat::mls, Δ{oriC dnaA}::{oriN repN Ppen-2028-dnaN cat}, lacA::{Pspank-dnaA tet}
CAS221	Δ{oriC dnaA}::{oriN repN Ppen-2028-dnaN cat}, amyE::{Pspank-dnaA spc}
CAS231	Δ{oriC dnaA}::{oriN repN Ppen-2028-dnaN cat}, amyE::{Pspank-dnaAΔC spc}
HM57	rok::pDG641rok (mls) (allele name rok57) (Smith & Grossman, 2015)
TAW5	trp+, Δ{oriC dnaA dnaN}::spc, spoIIIJ::{oriN repN kan}, amyE::(PxylA-dnaN cat), lacA::(Pspank-dnaA tet) (Merrikh & Grossman, 2011)
WKS1038	Δrok::cat (allele name rok1030) (Smits & Grossman, 2010)

All strains are derived from AG174 and contain the trpC2 pheA1 alleles (not listed) unless otherwise indicated.