Table 3.

Composition of the LAMP reaction mix

	Components	Volume
Reaction mix	20 mM Tris–HCl (pH 8.8)	12.5 µL
	10 mM KCl
	8 mM MgSO4
	10 mM (NH4)2SO4
	0.1% Tween-20
	1 mM Betaine
	1.6 mM dNTP
Primer mix	FIP (1.6 µM) and BIP (1.6 µM)	1 µL
	F3 (0.2 µM) and B3 (0.2 µM)
	FLF (0.8 μM) and FLB (0.8 μM)
Nuclease-free water		8 µL
Bst DNA polymerase		1 µL
SYTO-9		0.5 µL
DNA template		2 µL
Total volume		25 µL

Reaction condition: 63 °C, 45–60 min