Table 2.
Analysis of an angiogenesis RT-PCR gene expression array following Ang-(1-7) stimulation of rat microvascular endothelial cells
| Gene | Protein Annotation | Fold Regulation* |
p-value | Signaling Pathway Involvement* |
|---|---|---|---|---|
| Cdc42 | Cell division control protein 42 homolog (CDC42) | 1.22 | 0.047 | Rho GTPase, p21 signaling |
| Flt-1 | Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) | 3.02 | 0.050 | VEGF(+), PLCG(+), PKC(+), MAPK/ERK(+), AKT1(+) signaling |
| Flt-4 | Vascular Endothelial Growth Factor Receptor 4 (VEGFR4) | −5.97 | 0.050 | VEGF(+), PLCG(+), PKC(+), MAPK/ERK(+), AKT1(+) signaling |
| Hspb1 | Heat shock protein beta-1 (HSPB1) | 2.25 | 0.015 | Chaperone, thermotolerance, apoptosis(−), NF-κB(+) |
| Kdr | Vascular endothelial growth factor receptor 2 (VEGFR2) | 7.65 | 0.011 | angiogenesis(+), mitogenesis(+), cell migration(+) signaling |
| Map2k2 | Dual specificity mitogen-activated protein kinase kinase 2 (MEK2) |
1.57 | 0.005 | MAPK/ERK(+) signaling |
| Mapk1 | Mitogen-activated protein kinase 1 (MAPK1) | −1.16 | 0.031 | integration of signaling |
| Mapk12 | Mitogen-activated protein kinase 12 (MAPK12) | 2.57 | 0.017 | p38 MAPK, inflammatory(+) signaling |
| Mapk13 | Mitogen-activated protein kinase 11 (MAPK13) | 5.57 | 0.002 | p38 MAPK, ATF2(+), ELK1(+), PRKD1(−) signaling |
| Pgf | Placental Growth Factor (PGF) | −4.38 | 0.004 | FLT-1(+) signaling |
| Pik3cb | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PI3K beta isoform) |
−1.46 | 0.007 | PIP3(+), AKT1(+), PDPK1(+) signaling |
| Pik3cd | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PI3K delta isoform) |
−2.68 | 0.044 | PIP3(+), AKT1(+), PDPK1(+), RAS(+), MAPK/ERK(+), PI3K(+) signaling |
| Pik3cg | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform (PI3K gamma isoform) |
1.28 | 0.022 | PIP3(+), AKT1(+), PDPK1(+), cAMP(−), beta-adrenergic(−) signaling |
| Pla2g12a | Group XIIA secretory phospholipase A2 | 1.65 | 0.007 | phospholid(+), PKC(+), inflammatory(+) signaling |
| Pla2g2e | Group IIE secretory phospholipase A2 | 2.73 | 0.002 | phospholid(+), PKC(+), inflammatory(+) signaling |
| Pla2g4a | Cytosolic phospholipase A2 | −1.46 | 0.014 | phospholid(+), PKC(+), inflammatory(+) signaling |
| Ppp3ac | Calcineurin subunit B type 2 (CANB2) | 2.37 | 0.027 | calcium signaling(+) |
| Prkca | Protein kinase C alpha (PKCα) | −2.19 | 0.003 | calcium(+), DAG(+), RAF1(+), MAPK/ERK(+), proliferation(−), apoptosis(−) signaling |
| Ptgs2 | Prostaglandin G/H synthase 2 (cycloxygenase-2, COX-2) | −4.59 | 0.043 | prostaglandin signaling (+) |
| Ptk2 | Focal adhesion kinase 1 | 1.76 | 0.033 | migration(+), PI3K(+), AKT1(+), MAPK/ERK(+), Rho GTPase signaling |
| Rac2 | Ras-related C3 botulinum toxin substrate 2 | 6.65 | 0.009 | Rho GTPase signaling |
| Sh2d2a | SH2 domain-containing protein 2A (SH2D2A) | 7.18 | 0.017 | VEGFR2(+), MAPK(+), PKC(+) IKK/NFkB(+) signaling |
| Sphk1 | Sphingosine kinase 1 (SPHK1) | 1.78 | 0.023 | sphingosine(+), TNFα(+), NF-κB(+) signaling |
| Sphk2 | Sphingosine kinase 2 (SPHK2) | 1.62 | 0.045 | sphingosine(+), DAG(+), PKC(+), VEGF/MAPK/RAS(+) signaling |
| Vegfa | Vascular endothelial growth factor A (VEGF-A) | −2.93 | 0.014 | FLT1(+), KDR(+) signaling |
Note: All genes indicated significant fold change in gene expression (p≤0.05); biologic processes include but are not limited to those above (N=3).
*
Indicates 100 nM Ang-(1-7) stimulated versus unstimulated endothelial cells.