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. 2017 Feb 24;8:105. doi: 10.3389/fphys.2017.00105

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Copyright © 2017 Chung and Marshall.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparisons between magnetic resonance histology and conventional histology of a squid, Idiosepius notoides. (A,B) Transverse sections of squid head. Four different retinal layers can be identified, including the outer segment layer (Os), basal membrane (Bm), inner segment layer (Is), and cartilaginous eye cap (C). (C,D) Horizontal sections of squid head. (E,F) Sagittal sections of squid head. (G–I) Rhinophore. (J–L) Chromatophore. (E) indicates eye, gill (G), liver (L), ventral lobe (V), brachial lobe (Bl), statocyst (Sc), inferior buccal lobe (Ibl), optic lobe (Opl), pedal lobe (Pl), superior buccal lobe (Sbl), subesophageal mass (Sub), supraesophageal mass (Sup). The resolution of MRI slice at 16.4T (see Methods) is close to that of standard histology, however in contrast to histological sections that reveal cellular details, no individual cell can be identified in MRI images. Voxel resolution: 9 μm.