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. 2017 Feb 24;10:41. doi: 10.3389/fnmol.2017.00041

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Copyright © 2017 Hegle, Frank, Berndt, Klose, Allan and Accili.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ih disruption does not modify synaptic morphology in larval motor terminals. (A) Confocal images of the muscle 4 neuromuscular junction in wild type (left) and Ih^IE/Ih^RB (right) third instar larvae. Neuronal membranes were labeled with α-HRP (white) and synaptic active zones were labeled with α-Bruchpilot. Scale bars = 20 μm. (B) Histograms comparing the number of branches (left) and boutons (right) at the larval neuromuscular junction. No significant differences in branch or bouton number were observed between alleles (p > 0.05) using one-way ANOVA with Tukey's multiple comparisons test.