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The experimental process of Agrobacterium-mediated transformation using half-seed soybean explants. (A) Seed sterilization. (B) Seed imbibition. (C) Preparation of Agrobacterium. (D) Agrobacterium infection of half-seed cotyledonary explants. (E) Co-cultivation. (F,G) Shoot induction. (H) Shoot elongation. (I) Rooting. (J,K) Transplanting and adaptation to normal growth condition.
