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. 2017 Feb 24;8:246. doi: 10.3389/fpls.2017.00246

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Copyright © 2017 Li, Cong, Liu, Wang, Shuai, Chen, Gai and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of DTT on the transient GUS expression in soybean cotyledonary explants. (A) Rate of total GUS transient expression in Jack Purple explants. (B) Rate of total GUS transient expression in Tianlong 1 explants. (C) Rate of strong GUS transient expression in Jack Purple explants. (D) Rate of strong GUS transient expression in Tianlong 1 explants. Results are expressed as mean ± standard error. Fifty explants were stained for each treatment and the experiments were repeated twice. ^* above bars indicates significant difference between 0 and 154.2 mg/L DTT at 0.05 level by student's t-test. Rate of total GUS transient expression (%) = (The number of explants with deep and weak dyeing / Total number of explants for staining) × 100%. Rate of strong GUS transient expression (%) = (The number of explants with deep dyeing/Total number of explants for staining) × 100%.