Figure 6.

The effect of different explants on the transient GUS expression. The cotyledonary explants were obtained by four different methods, including imbibition for 1 d, germination for 1, 3, and 5 d. (A) Rate of total GUS transient expression in the cotyledonary explants of Jack Purple. (B) Rate of total GUS transient expression in the cotyledonary explants of Tianlong 1. The results are expressed as mean ± standard error. Fifty explants were stained for each treatment and the experiments were repeated twice. Means with the same letter above bars are not significantly different at 0.05 level according to Duncan's multiple range test. Rate of total GUS transient expression (%) = (The number of explants with deep and weak dyeing/Total number of explants for staining) × 100%.