. 2017 Feb 24;8:246. doi: 10.3389/fpls.2017.00246

Table 1.

The effect of combination of different GA₃ and IAA concentrations on the rate of shoot elongation and transformation efficiency in soybean.

Soybean variety	GA₃ concentration (mg/L)	IAA concentratio (mg/L)n	Rate of shoot elongation (%)	Transformation efficiency (%)
Jack Purple	0.5	0.1	16.11 ± 0.05^b	5.00 ± 0.03^a
	0.5	0.2	13.70 ± 0.02^b	4.57 ± 0.01^a
	1.0	0.1	33.54 ± 0.03^a	7.32 ± 0.01^a
	1.0	0.2	17.42 ± 0.01^b	5.26 ± 0.01^a
	1.5	0.1	11.49 ± 0.01^b	5.41 ± 0.03^a
	1.5	0.2	13.39 ± 0.05^b	5.36 ± 0.01^a
Tianlong 1	0.5	0.1	14.75 ± 0.01^ab	4.28 ± 0.00^a
	0.5	0.2	10.60 ± 0.01^b	1.76 ± 0.02^a
	1.0	0.1	26.08 ± 0.07^a	10.01 ± 0.03^a
	1.0	0.2	9.80 ± 0.04^b	3.53 ± 0.02^a
	1.5	0.1	19.56 ± 0.05^ab	4.49 ± 0.03^a
	1.5	0.2	14.40 ± 0.03^ab	5.69 ± 0.04^a

The results are expressed as mean ± standard error. Eighty explants were infected by Agrobacterium for each combination/treatment and the experiments were repeated twice. The concentrations of glufosinate for selection were 5 mg/L and 3 mg/L in SIM and SEM, respectively. The numbers of elongated shoots (height ≥ 3 cm) were recorded during SEM stage. For each soybean variety, means with the same letter are not significantly different at 0.05 level according to Duncan's multiple range test. Rate of shoot elongation (%) = (The number of elongated shoots/the number of infected explants) × 100%. Transformation efficiency (%) = (The number of the positive plants/the number of infected explants) × 100%.