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Comparison of different methods to detect positive transgenic soybean plants at T0 generation.
| Method | Number of positive plants/Total plants | Time after shoot elongation | Time needed for testing |
|---|---|---|---|
| GUS staining during rooting | 20/20 | 1 day | Overnight |
| GUS staining after transplanting | 16/20 | 2 weeks | Overnight |
| PCR amplification of GUS gene | 20/20 | 3–4 weeks | 2 h |
| Glufosinate painting on leaves | 20/20 | 4 weeks | 7 days |
| LibertyLink® strip detection | 20/20 | 4 weeks | 10 min |
