Table 5.

The copy number of bar gene in T₁ transgenic soybean plants.

Samplea	Cbt,X	Cct,R	Ratio of bar/lectin (X0/R0)d	Copy number of bar gene
1	26.94 ± 0.12	20.52 ± 0.00	3.10	3
2	26.24 ± 0.39	20.78 ± 0.56	1.55	2
3	26.74 ± 0.11	20.17 ± 0.21	1.42	1
4	25.51 ± 0.24	21.58 ± 0.09	1.56	2
5	24.39 ± 0.17	21.52 ± 0.23	3.42	3
6	26.54 ± 0.08	21.85 ± 0.15	0.88	1

^a1–3, positive T₁ transgenic soybean plants in Jack Purple background. 4–6, positive T₁ transgenic soybean plants in Tianlong 1 background.

^bC_t,X represents the Ct value of the exogenous target gene (bar).

^cC_t,R represents the Ct value of the internal reference gene (lectin).

^dX₀/R₀ is the ratio of initial amount of bar/lectin. X₀/R₀ = 10^{[(Ct, X−IX)/SX]−[(Ct, R−IR)/SR]}, where IX and SX is the intercept and slope of the standard curve of the target bar gene, respectively, and IR and SR is the intercept and slope of the standard curve of the reference lectin gene, respectively.