Table 1.
Amino acid | WT | PCAS1 | PCAS2 |
---|---|---|---|
CyA | 1.30 ± 0.05 | 1.49 ± 0.19 | 1.40 ± 0.08 |
His | 0.56 ± 0.17 | 0.7 ± 0.22 | 0.7 ± 0.2 |
Ser | 1.63 ± 0.4 | 2.08 ± 0.42 | 2.19 ± 0.28a |
Arg | 1.30 ± 0.21 | 2.68 ± 0.54a | 1.83 ± 0.25ab |
Gly | 4.01 ± 0.18 | 4.87 ± 1.01 | 5.56 ± 0.45a |
Asp | 6.56 ± 0.78 | 9.12 ± 0.92a | 8.43 ± 0.86a |
MetS | 1.64 ± 0.25 | 1.74 ± 0.3 | 1.86 ± 0.11 |
Glu | 7.27 ± 0.35 | 9.76 ± 1.07a | 9.92 ± 0.88a |
Thr | 1.62 ± 0.32 | 1.98 ± 0.51 | 2.25 ± 0.15a |
Ala | 3.93 ± 0.28 | 4.49 ± 1 | 4.96 ± 0.34a |
Pro | 2.05 ± 0.17 | 2.41 ± 0.56 | 2.67 ± 0.3 |
Lys | 3.01 ± 0.16 | 3.62 ± 0.69 | 3.87 ± 0.19a |
Val | 2.78 ± 0.19 | 3.32 ± 0.74 | 3.64 ± 0.28a |
Ile | 2.01 ± 0.11 | 2.35 ± 0.48 | 2.58 ± 0.17a |
Leu | 3.0 ± 0.16 | 3.51 ± 0.73 | 3.89 ± 0.26a |
Phe | 1.62 ± 0.09 | 1.78 ± 0.32 | 1.86 ± 0.14a |
Total | 44.3 | 55.9a | 57.6a |
Superscript ‘a’ indicates significant difference from wild-type at P ≤ 0.05. Superscript ‘b’ indicates significant difference between the two transgenic lines at P ≤ 0.05. Values are ± standard deviation. CyA, cysteic acid (hydrolysis product of cysteine); Asp, Asp+Asn (due to hydrolysis of Asn to Asp); Glu, Glu+Gln (due to hydrolysis of Gln to Glu).