Table 1.
The primer sequences used for real-time qPCR of RNA obtained from the eggs of European sea bass characterized by different quality
| Symbol | Forward sequence | Reverse sequence |
|---|---|---|
| Differentially expressed genes | ||
| polk | GCAAAGAAACTCTGCCCCAA | CCGGATCTCATTGCACACAG |
| rab2a | GGCTTTCATCAACACAGCCA | GGAGTTGGTGGTAGGATGCT |
| irf7 | CTAACCGTCTCCAGCTCCAT | CAGTGGATGGGAAGCAGAGA |
| usp5 | AGAAGGATCAGCAGTGGGTC | GCTCCCTCCCTTGTCTCATT |
| kctd12 | GGAGTGTGCTTGCATTGTCA | CTTATCCACCACACCCCTCA |
| plec | TCCTCTCAGTCCAGCAAAGG | ATCCTGTCCTTGAGCCAGTC |
| hy-prot(1) | GGTAGCCCTGCCCTTCTTAA | TTAGTGGGCTGCATCCTCAA |
| mem-prot | ACGTTCTGTCGTGCCTCTCT | TGCAGAGGGCTTTTGCTATT |
| Most stable genes | ||
| hspa14 | GTGCCTGAGGAAGAGTCTGT | CCCGATGGAAGGAGAACAGT |
| cops3 | GCCTTGGAGCAGTTTGTGAA | TTGATCAACTCGCACAGCTG |
| mtif3 | CACCATGCACCGTCTAGTTG | TTTGTGTTCACTGACCAGCG |
| eif3ea | CTCACCACCAAAATTGCCCA | GCGAAGTCCACCATGTTTGT |
| hectd3 | AGCCGCACTCAAAGGAAAAG | CAGAGTCTACAGCGGGGAAA |
| eif3e | AGAGCACCAAGAACGAGACA | TCCATCTTGCTTGAGTCGCT |
Differentially expressed genes refer to the ones exhibiting different mRNA abundance between the groups representing “low” and “high” egg quality. Most homogenized genes are the ones used for the data normalization. For each gene symbol, the protein is provided according to the iHOP database or specific abbreviation was introduced (explained in the footnote of the table). Numbers in parenthesis represent number of subsequent gene, when more than one gene with the same name was identified
hy-prot hypothetical protein, un-prot uncharacterized protein, n-f protein was not found, mem-prot membrane protein