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. 2016 Oct 6;21(3):568–578. doi: 10.1111/jcmm.13002

Figure 1.

Figure 1

Leptin induces GATA3 expression. (A and B) Detection of GATA3 expression at protein and mRNA levels (n = 3). Serum‐starved HSCs were incubated with or without increased doses of leptin (A) or 100 ng/ml of leptin (or the vehicle, −) (B) for 24 hrs and GATA3 protein and mRNA levels were examined by western blot analyses and real‐time PCR, respectively. *P < 0.05. (C) Examination of GATA3 promoter activity by luciferase assay (n = 3). HSCs were transfected with 1.6 μg of GATA3 promoter reporter plasmid pGATA3(−2531)Luc and then underwent 12 hrs of serum starvation before addition of 100 ng/ml of leptin (or the vehicle, −) for another 24 hrs. Luciferase assay was performed. *P < 0.05.