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. 2016 Oct 19;4(2):1600327. doi: 10.1002/advs.201600327

Figure 2.

Figure 2

Uptake of HGN‐siHsp70 in U87MG cells intracellular fate of siRNAs after endocytosis. a) Cellular uptake of HGN‐siHsp70 within 120 min measured by dark field and laser confocal microscopy. b) Dark field images of U87MG and MDA‐MB‐231 cells after 120 min incubation with HGN, HGN conjugated with nonsense sequences (HGN‐N), and HGN‐siHsp70. c) Quantification and comparison of gold content in cells treated with HGN with and without oligonucleotide modification using ICP‐AES. d) Laser confocal images of the uptake of HGN‐siHsp70 in U87MG cells pretreated with different chemical inhibitors. e) Endosomal escape of HGN‐siHsp70 after laser treatment, red: lysosome, green: HGN‐siRNA, and yellow: colocalization of HGN‐siHsp70 and endolysosome.