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. 2004 Nov 30;2(12):e418. doi: 10.1371/journal.pbio.0020418

Figure 3. Effects of Salmonella Virulence Mutations on Disease in the Fly.

Figure 3

(A) Mean time to death for infected flies. Identical quantities of S. typhimurium strains were injected into Oregon R flies and survival was monitored daily (i). Infections with slrP and rescuing construct were performed separately and are thus reported separately (ii).

(B) Growth of mutant Salmonella in the fly. Approximately 10,000 cfu of each bacterial strain were injected into flies. Flies were then homogenized and plated at the time of injection (black bars) or following a 7-d incubation (white bars). All error bars show standard deviation.

(C–H) Phagocytosis assays in living Drosophila. To assay the effects of Salmonella infections on phagocyte function, flies were injected with approximately 10,000 cfu of each strain of S. typhimurium. Following a 7-d incubation, the flies were first injected with FITC-labeled dead E. coli and incubated for 60 min to permit them to be phagocytosed. Trypan blue was then injected to quench the fluorescence of extracellular bacteria. The area boxed in (C) was photographed using FITC optics (D–H). The flies were injected with the following bacterial strains: (D) SL1344; (E) LB (control); (F) BJ66 (SPI1); (G) P3F4 (SPI2); (H) slrP (Table 1).