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. 2017 Jan 12;6(3):267–275. doi: 10.1016/j.molmet.2017.01.002

Figure 1.

Figure 1

Inducible adipose-specific overexpression of AdipoR1/2 results in systemic improvements in glucose and lipid homeostasis. A) Analysis of neutral ceramidase activity from adipose depots and liver of Art-R1, Art-R2 mice, and WT littermates after 10 days of dox-Chow. B) After 8 weeks of HFD, mice were switched to HFD-dox. Circulating glucose levels were measured during an oral glucose tolerance test (OGTT, 2.5 g/kg glucose per oral gavage) 7 days after dox. C) Serum glucose levels during an ITT (0.75 U/kg, IP) of Art-R1 mice and WT littermates. D) Circulating glucose levels measured during an ITT (0.75 U/kg, IP) of Art-R2 mice and WT littermates. E) Glucose infusion rates (left) and hepatic glucose output (right) during hyperinsulinemic-euglycemic clamp experiments performed on conscious unrestrained 20-week-old WT and Art-R2 males or 15-week-old WT and Art-R1 males F) Circulating triglyceride (TG) levels were measured during an oral TG clearance test (20% intralipid, 15 uL/g body weight; single gavage) in Art-R1, Art-R2, and WT littermate controls. G) Hepatic TG content was enzymatically quantified. H-J) Ceramide species of the indicated chain lengths were quantified in mesenteric and inguinal adipose depots (H), serum (I) and liver (J) expressed as % of WT. All samples are from Art-R1, Art-R2 mice, and WT littermates after 8 weeks of HFD-dox challenge (n = 6–8/group), unless specified otherwise. * corresponds Art-R1 compared to WT littermates and # corresponds to Art-R2 compared to WT littermates. For Figure I, * corresponds to Art-R1 mWAT compared to WT mWAT, # corresponds to Art-R2 mWAT compared to WT mWAT, and & corresponds to Art-R2 sWAT compared to WT sWAT. *, #, &: P < 0.05.