TABLE 2.
β-Galactosidase activity of the different CV1008 derivatives (ilvI′-lacZ)a
| Strain | β-Galactosidase activity (%)
|
|
|---|---|---|
| Without amino acid | +Leucine, 1.5 mM | |
| FB3002 (Lrp) | 100.0 ± 19.2 | 17.4 ± 4.2 |
| FB3003 (LrpS41P) | 20.4 ± 5.9 | 18.0 ± 3.6 |
| FB3004 (LrpL131R) | 218.5 ± 37.7 | 174.3 ± 29.3 |
| FB3005 (LrpL131A) | 126.9 ± 2.4 | 161.6 ± 27.5 |
| FB3006 (LrpL136R) | 102.4 ± 18.6 | 83.8 ± 16.2 |
| FB3007 (LrpV149A) | 107.2 ± 15.6 | 48.0 ± 7.2 |
a
The arabinose concentration used was 50 μg/ml. Specific activities obtained were normalized to that of FB3002.