TABLE 3.
β-Galactosidase activity of the different FB1001 and FB2001 derivativesa
| Strain | β-Galactosidase activity (Miller units)
|
||
|---|---|---|---|
| Without amino acids | +Leucine, 1.5 mM | +Alanine, 1.5 mM | |
| FB1002 (Lrp) | 107.7 ± 9.8 | 28.3 ± 2.4 | 34.2 ± 2.7 |
| FB1003 (LrpS41P) | 12.2 ± 3.2 | 14.0 ± 3.7 | 14.8 ± 3.3 |
| FB1004 (LrpL131R) | 132.5 ± 2.8 | 147.2 ± 5.2 | 135.9 ± 2.6 |
| FB1005 (LrpL131A) | 109.0 ± 13.7 | 92.4 ± 8.5 | 121.6 ± 19.6 |
| FB1006 (LrpL136R) | 89.4 ± 2.2 | 105.1 ± 2.0 | 98.9 ± 4.0 |
| FB1007 (LrpV149A) | 93.8 ± 2.1 | 66.0 ± 5.3 | 74.3 ± 2.9 |
| FB1008 (LrpE133G) | 17.4 ± 3.8 | 14.1 ± 1.2 | 17.6 ± 4.1 |
| FB2001 (Lrp) | 75.5 ± 6.6 | 17.6 ± 2.7 | 22.0 ± 0.9 |
| FB2006 (LrpL136R) | 61.7 ± 8.7 | 62.5 ± 6.3 | 63.5 ± 5.7 |
a
The arabinose concentration used was 50 μg/ml. The post-hoc test of Tukey was used to analyze the effect of the medium on the expression of foo (9).