TABLE 1.
Bacterial strains used in this study
| Salmonella enterica serovar Typhi- murium strain | Parental strain | Genotype or phenotype | Derivation or source (reference) |
|---|---|---|---|
| ATTC 14028 | 14028 | Wild type | ATTC |
| KR1657 | recA1 zfi-1623::Tn10d-Camr | Salmonella Genetic Stock Centre, Calgary, Alberta, Canada | |
| TT14282 | srl-1203::Tn10d-CamrrecA1 | Salmonella Genetic Stock Centre, Calgary, Alberta, Canada | |
| YS1 | 14028 | msbB1::ΩTetr | P22 · YS8211 × 14028 → Tet5r, where YS8211 = donor and 14028 = recipient in P22 transduction (18) |
| YS871 | 14028 | ΔmsbB2 Δpur13252 somA+zbj-10::Tn10 | P22 · 14028 Tn10 pool × YS1456 → Tet20r (EGTAs) |
| YS1124 | 14028 | ΔmsbB2 ΔpurI3252 Suwwan deletion | Spontaneous EGTAr derivative of msbB1::ΩTet ΔpurI3252 derivative of 14028; replacement of msbB1::ΩTetr with ΔmsbB2 by homologous recombination (9) |
| YS1170 | 14028 | msbB1::ΩTetr Suwwan deletion | YS8211; spontaneous selection on EGTA medium |
| YS1317 | 14028 | ΔmsbB2 ΔpurI3252 somA+zbj-10::Tn10 | P22 · YS871 × YS1456 → Tet20r (EGTAs) |
| YS1456 | 14028 | ΔmsbB2 ΔpurI3252 somA1 | Spontaneous EGTAr derivative of msbB1::ΩTetr ΔpurI3252 derivative of 14028; replacement of msbB1::ΩTetr with ΔmsbB2 by homologous recombination (9, 18) |
| YS1770 | 14028 | somA2::Kanr | Lambda red system used to integrate somA2::Kanr PCR product (5) |
| YS1920 | 14028 | msbB1::ΩTetr Suwwan deletion | YS1; spontaneous selection on EGTA medium |
| YS1925 | 14028 | msbB1::ΩTetr Suwwan deletion | YS1; spontaneous selection on EGTA medium |
| YS2049 | 14028 | somA2::Kanrzbj-10::Tn10 | P22 · YS871 × YS1770 → Kan5r |
| YS2062 | 14028 | Suwwan deletion | Bochner selection (3) of YS2049 |
| YS2200 | 14028 | ΔmsbB2 ΔpurI3252 somA+zbj-10::Tn10 srl-203::Tn10d-CamrrecA1 | P22 · TT14282 × YS1317 → Cam20r |
| YS2201 | 14028 | ΔmsbB2 Δpur13252 somA+zbj-10::Tn10 recA1 zfi-1623::Tn10d-Camr | P22 · KR1657 × YS1317 → Cam20r |
| YS2202 | 14028 | somA2::Kanrzbj-10::Tn10 recA1 srl::Tn10d-Camr | P22 · TT14282 × YS2049 → Cam20r |
| YS8211 | 14028 | msbB1::ΩTetr | Low et al. (14) |
| TT16812 | LT2 | recD541::Tn10dCmr | Salmonella Genetic Stock Center, Calgary, Alberta, Canada; used as a representative Salmonella LT2 strain |
| YS2 | LT2 | msbB1::ΩTetrrecD541::Tn10dCmr | P22 · YS8211 × TT16812 → Tet5r (18) |
| YS2050 | LT2 | ΔmsbB2 zbj-10::Tn10 recD541::Tn10dCmr | P22 · YS871 × YS2 → Tet20r |
| YS2057 | LT2 | recD541::Tn10dCmrsomA2::Kanr | P22 · YS1770 × TT16182 → Kan5r |
| YS2087 | LT2 | recD541::Tn10dCmrsomA2::Kanrzbj-10::Tn10 | P22 · YS871 × YS2057 → Tet20r |
| YS2107 | LT2 | recD thy somA2::Kanrzbj-10::Tn10 | Trimethoprim selection (8) of YS2087 |
| YS2111 | LT2 | recD somA2::Kanrzbj-10::Tn10 | P22 · 14028 × YS2107 → Thy+ |
| SL1344 | SL1344 | his Strr; mouse virulent | Sunshine et al. (24) |
| YS3 | SL1344 | msbB1::ΩTetr | P22 · YS8211 × SL1344 → Tet5r (18) |
| YS2051 | SL1344 | msbB1::ΩTetrzbj-10::Tn10 | P22 · YS871 × YS3 → Tet20r |
| YS2058 | SL1344 | somA2::Kanr | P22 · YS1770 × SL1344 → Kan5r |
| YS2096 | SL1344 | somA2::Kanrzbj-10::Tn10 | P22 · YS1770 × YS2058 → Kan5r |