TABLE 2.
Frequencies of suppressor formation
| Strain | Avg no. of CFU/ml on MSB agar | Suppressor mutation frequency | Suwwan deletion mutation frequency | % of suppressed strains with Suwwan deletion |
|---|---|---|---|---|
| YS1317 (msbB 14028) EGTA selection | 7.9 × 106 | 3.1 × 10−4 | 1.0 × 10−4 | 44 |
| YS1317 (msbB 14028) Mac-Conkey galactose selection | 7.9 × 106 | 5.4 × 10−4 | 9.2 × 10−5 | 29 |
| YS2050 (msbB recD LT2) EGTA selection | 2.3 × 107 | 1.9 × 10−4 | 0 | 0 |
| YS2050 (msbB recD LT2) Mac-Conkey galactose selection | 2.3 × 107 | 4.3 × 10−6 | 0 | 0 |
| YS2051 (msbB SL1344) EGTA selection | 3.6 × 107 | 1.1 × 10−4 | 0 | 0 |
| YS2051 (msbB SL1344) Mac-Conkey galactose selection | 3.6 × 107 | 6.9 × 10−5 | 0 | 0 |
| YS2200 (recA1 msbB 14028) EGTA selection | 8.3 × 106 | 2.5 × 10−4 | 0 | 0 |
| YS2200 (recA1 msbB 14028) Mac-Conkey galactose selection | 8.3 × 106 | 1.3 × 10−4 | 0 | 0 |
| YS2201 (recA1 msbB 14028) EGTA selection | 1.3 × 107 | 9.8 × 10−3 | 0 | 0 |
| YS2201 (recA1 msbB 14028) Mac-Conkey galactose selection | 1.3 × 107 | 6.1 × 10−4 | 0 | 0 |