FIG. 1.

Profiles of PEP-dependent activity (○) and TP activity (□) as well as protein concentration (Δ) following gel filtration of a 2-h HSS from an extract of E. coli strain ZSC112L(pJFGH11). Elution was with M63, pH 7.0, containing 2 mM dithiothreitol. [¹⁴C]methyl-α-glucoside (20 μM for the PEP reaction; 100 μM for the TP reaction) plus either PEP (10 mM) and a 4-h HSS or glucose-6-P (10 mM) were used for the assay under standard conditions. The fractions pooled for enzyme analysis are shown by the horizontal black bars.