FIG. 3.

TP activities of Ni²⁺ column-purified preparations of the II^Glc from gel filtration peaks 1 (Δ) and 2 (○). The enzyme preparation applied to the gel filtration column was a 2-h HSS from strain ZSC112L(pJFGH11). Activity was measured as a function of glucose-6-P concentration. The final methyl-α-glucoside concentration was 50 μM. The specific activities, when measured with a final [¹⁴C]methyl-α-glucoside concentration of 50 μM and a final glucose-6-P concentration of 5 mM with 1 μg of phosphatidylglycerol/μl present, were 16 and 0.7 pmol of sugar-P formed per mg of protein per min for the peak 1 and peak 2 enzyme preparations, respectively.