FIG. 5.

TP activities of the detergent-solubilized pellet fraction (Δ), peak 1 of the solubilized pellet fraction eluted with M63, pH 8.9, containing 0.1% Triton X-100 (○), and peak 2 of the solubilized pellet fraction eluted with M63, pH 8.9, containing 0.1% Triton X-100 (×). All three fractions were dialyzed three times against 2 liters water for 12 h each before assay. The assay was performed as a function of glucose-6-P concentration with the [¹⁴C]methyl-α-glucoside concentration held constant at 50 μM. Phosphatidylglycerol dissolved in hexane-ethanol (9:1) was included in the assay mix at a final concentration of 1 mg/ml. The specific activities of the peak 1 and peak 2 enzyme preparations were 36 and 20 pmol of sugar-P formed per mg of protein per min, respectively, with [¹⁴C]methyl-α-glucoside at 50 μM and glucose-6-P at 5 mM.