TABLE 1.

Description of strains used in this study

Strain	Description	Reference or source	Use in this study
S301	An lpp-2 derivative of W3110, wild type with regard to phospholipid synthesis	17	Kinetics study of soluble versus membrane enzymes II
AD90	A pssA::kan mutant strain derived from strain AD90/pDD72 by plasmid curing; the latter strain was derived from strain W3899	W. Dowhan (6)	4-h HSS was used as a source of soluble PTS enzymes (enzyme I, enzyme IIAGlc, and HPr)
ZSC112L(pJFGH11) (Ampr)	Carries plasmid pJFGH11 with IICBGlc with a C-terminal His tag; the chromosomal ptsG gene is inactivated	B. Erni (48)	Western blot study of EIIGlc using monoclonal antibody against His tag
ZSCΔmanXYZ (pTSP-H6M) (Cmr Ampr)	Carries plasmid pTSP-H6M (IICMan::H6IIDMan) with His tag at the N terminus of IIDMan; the chromosomal man operon is deleted	B. Erni (10)	Western blot study of EIIMan using monoclonal antibody against His tag