Table 1. The differences in pigment composition and chlorophyll fluorescence parameters of wild-type and npq4 cells after the high light pre-treatment.
WT | SD | npq4 | SD | % from WT | |
---|---|---|---|---|---|
Lutein | 15.06 | 1.32 | 16.03 | 0.89 | +6.4 |
Total VAZ | 9.09 | 0.43 | 10.31 | 0.77 | +13.4* |
Chl. a:b | 2.49 | 0.11 | 2.70 | 0.08 | +8.5* |
Fv/Fm | 0.63 | 0.01 | 0.61 | 0.01 | −3.8* |
Fo (DCMU) | 0.100 | 0.004 | 0.107 | 0.003 | +7.0 |
‘WT’ = wild-type, ‘Total VAZ’ = total xanthophyll pool of violaxanthin, antheraxanthin and zeaxanthin calculated on a mol basis and expressed as molx100:mol total chlorophyll, as for lutein. ‘Chl. a:b’ = ratio of chlorophyll a:b calculated on a mol basis, ‘Fv/Fm’ = maximum quantum yield of PSII, ‘Fo (DCMU)’ = Fo measured in cultures at 10 μg mL−1 chlorophyll in the presence of 10 μM DCMU, ‘% from WT’ was calculated as (npq4-WT)/WT × 100 and ‘*’ corresponds to a significant difference (P < 0.05), n = 4 ± SD.