Table 1. Inflammatory cytokine and chemokine levels in the lungs following CPI-203 treatment in a murine model of Th17-induced allergic airway disease.
| Control (n = 5) | CPI-203 (n = 6) | Th17 (n = 8) | Th17+CPI-203 (n = 8) | |
|---|---|---|---|---|
| IL-1α | 22.57 ± 1.97 | 22.81 ± 1.00 | 142.75 ± 4.33* | 166.47 ± 8.83* |
| IL-1β | 233.89 ± 14.18 | 240.68 ± 5.64 | 2067.55 ± 37.30* | 2415.43 ± 85.61* |
| IL-2 | 55.99 ± 4.25 | 58.81 ± 2.25 | 189.90 ± 7.53* | 248.55 ± 13.99* |
| IL-3 | 11.50 ± 0.80 | 11.32 ± 1.24 | 15.05 ± 1.31 | 19.24 ± 2.29† |
| IL-4 | 3.51 ± 0.14 | 3.57 ± 0.05 | 59.88 ± 7.47 | 98.92 ± 25.54† |
| IL-5 | 11.87 ± 0.99 | 13.05 ± 0.54 | 26.95 ± 2.55† | 33.30 ± 4.16† |
| IL-6 | 0.00 | 0.00 | 89.40 ± 3.40 | 108.37 ± 4.69‡ |
| IL-9 | 0.00 | 0.00 | 968.40 ± 33.54 | 1030.62 ± 30.50 |
| IL-10 | 24.35 ± 0.90 | 27.18 ± 0.67 | 373.72 ± 7.87* | 462.43 ± 17.65* |
| IL-12p40 | 78.45 ± 17.87 | 49.06 ± 15.88 | 261.17 ± 31.22* | 467.50 ± 44.09* |
| IL-12p70 | 96.28 ± 9.40 | 105.46 ± 5.66 | 510.79 ± 25.30* | 691.97 ± 34.71* |
| IL-13 | 281.83 ± 15.57 | 307.55 ± 15.96 | 1720.70 ± 74.47* | 2329.93 ± 150.05* |
| IL-17A | 8.41 ± 0.35 | 9.41 ± 0.53 | 90.03 ± 3.20* | 110.35 ± 6.73* |
| EOTAXIN | 467.54 ± 24.21 | 538.83 ± 28.85 | 2255.97 ± 99.10* | 2753.50 ± 87.87* |
| G-CSF | 0.00 | 0.00 | 227.80 ± 28.41 | 126.43 ± 13.84‡ |
| GM-CSF | 123.00 ± 3.81 | 134.20 ± 5.47 | 184.52 ± 4.30† | 186.91 ± 4.35† |
| IFNγ | 28.26 ± 2.96 | 34.22 ± 3.71 | 65.86 ± 5.07 | 206.78 ± 80.14 |
| CXCL1 | 32.33 ± 1.61 | 26.85 ± 1.56 | 346.10 ± 29.10* | 268.39 ± 17.61* |
| MCP-1 | 85.90 ± 8.74 | 90.21 ± 5.75 | 851.93 ± 64.59† | 1031.35 ± 99.39† |
| MIP-1α | 36.29 ± 1.95 | 30.04 ± 2.20 | 197.01 ± 12.89† | 178.24 ± 7.44† |
| MIP-1β | 51.48 ± 2.39 | 50.75 ± 1.69 | 126.71 ± 7.14* | 102.83 ± 6.50* |
| CCL5 | 45.90 ± 1.37 | 28.06 ± 1.72 | 680.98 ± 34.69* | 516.05 ± 27.07* |
| TNFα | 176.06 ± 5.68 | 194.75 ± 4.66 | 497.21 ± 10.94† | 567.25 ± 37.35† |
Values shown are mean ± SEM. Comparisons were made using an unpaired t-test (IL-6, IL-9, G-CSF) or an one-way ANOVA and Tukey’s post-test (all other analytes). Mice in control and CPI-203 groups received all ovalbumin challenges, but received phosphate buffered saline (PBS) retro-orbitally at time of cell transfer. *p < 0.05 when compared to all other groups, †p < 0.05 when compared to control and CPI-203 groups, and ‡p < 0.05 when compared to the Th17 group.