Table 1. Substrate specificity of the recombinant SALDan.
| Substrates | App Km (μM) | App Vmax (U/mg) | App kcat (s−1) | App kcat/Km (s−1 • μM−1) | App Ki (μM) |
|---|---|---|---|---|---|
| Salicylaldehyde | 3.8 ± 0.5 | 49.5 ± 4.5 | 123.6 ± 13.6 | 32.5 ± 4.2 | 378.7 ± 45.2 |
| Benzaldehyde | 2.3 ± 0.3 | 22.6 ± 3.8 | 56.1 ± 10.5 | 24.2 ± 2.9 | 562.6 ± 87.6 |
| 2-Chlorobenzaldehyde | 3.2 ± 0.3 | 45.6 ± 6.2 | 114.2 ± 17.2 | 35.7 ± 4.5 | 356.4 ± 40.1 |
| 3-Chlorobenzaldehyde | 4.8 ± 0.7 | 56.8 ± 7.2 | 142.1 ± 20.5 | 29.6 ± 3.5 | 123.9 ± 20.1 |
| 4-Chlorobenzaldehyde | 5.5 ± 1.1 | 74.2 ± 5.8 | 185.5 ± 16.1 | 33.7 ± 2.8 | 132.1 ± 18.9 |
| 4-Nitrobenzaldehyde | 6.5 ± 0.9 | 32.3 ± 4.6 | 80.2 ± 14.5 | 12.3 ± 2.0 | 265.7 ± 35.8 |
| 2-Naphthaldehyde | 3.4 ± 0.5 | 61.4 ± 5.1 | 153.1 ± 14.4 | 45.0 ± 4.4 | 248.5 ± 22.5 |
| Formaldehyde | 12360 ± 1820.0 | 3.3 ± 0.7 | 8.6 ± 2.2 | 7.0E−04 ± 8.4E−03 | — |
| Butyraldehyde | 3200 ± 235.0 | 5.9 ± 1.0 | 14.3 ± 2.8 | 4.5E−03 ± 5.7E−02 | — |
| Glutaraldehyde | 560 ± 46.0 | 11.6 ± 1.2 | 29.6 ± 3.7 | 5.3E−02 ± 4.9E−01 | — |
“—” means not observed.