Table 2. Apparent kinetic parameters of the wild-type and mutant SALDan enzymes.
| Proteins | Substrates | App Km (μM) | App Vmax (U/mg) | App kcat (s−1) | App kcat/Km (s−1 • μM−1) | App Ki (μM) |
|---|---|---|---|---|---|---|
| WT | NAD+ | 39.5 ± 3.2 | 94.1 ± 11.5 | 234.3 ± 24.2 | 5.9 ± 0.5 | — |
| SALD | 3.8 ± 0.5 | 49.5 ± 4.5 | 123.6 ± 13.6 | 32.5 ± 4.2 | 378.7 ± 45.2 | |
| N149A | NAD+ | 52.3 ± 6.1 | 83.5 ± 10.8 | 208.8 ± 25.6 | 4.0 ± 0.8 | — |
| SALD | 9.5 ± 1.3 | 38.6 ± 3.5 | 100.4 ± 16.8 | 10.6 ± 1.3 | 446.7 ± 52.6 | |
| V153A | NAD+ | 44.9 ± 4.2 | 102.6 ± 9.8 | 254.5 ± 21.5 | 5.7 ± 0.5 | — |
| SALD | 4.6 ± 0.7 | 59.5 ± 7.6 | 153.5 ± 20.4 | 33.4 ± 4.7 | 428.3 ± 49.4 | |
| E175A | NAD+ | 105.6 ± 10.3 | 60.6 ± 6.9 | 157.6 ± 17.2 | 1.5 ± 0.4 | — |
| SALD | 15.1 ± 2.6 | 32.3 ± 4.3 | 82.2 ± 10.1 | 5.4 ± 0.9 | 461.6 ± 51.4 |
“—” means not observed.