Table 1. Primer sequences for target and housekeeping genes.
Gene | Primer sequence | PCR product size |
---|---|---|
GAPDH | Forward: 5′-TTCCAGGAGCGAGATCCCT-3′ Reverse: 5′-CACCCATGACGAACATGGG-3′ | 175 bp |
CSF2RA | Forward: 5′-TGTCGCTCTTCCCTTCTCTC-3′ Reverse: 5′-TTCGCAGATCCGATTTCTCT-3′ | 116 bp |
CRLF2 | Forward: 5′-TGCACCAACTACCTTCTCCA-3′ Reverse: 5′-TGATGCCACGAAAATCTCAC-3′ | 182 bp |
IL3RA | Forward: 5′-TTAAGCAGGCACCTCTGTCC-3′ Reverse: 5′-CTGAGCCTTTGCTTTCATCC-3′ | 154 bp |
PCR, polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; CSF2RA, colony stimulating factor 2 receptor, alpha; CRLF2, cytokine receptor-like factor 2; IL3RA, interleukin 3 receptor, alpha.