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. 2017 Feb 24;7:43469. doi: 10.1038/srep43469

Table 1. Primer sequences for target and housekeeping genes.

Gene Primer sequence PCR product size
GAPDH Forward: 5′-TTCCAGGAGCGAGATCCCT-3′ Reverse: 5′-CACCCATGACGAACATGGG-3′ 175 bp
CSF2RA Forward: 5′-TGTCGCTCTTCCCTTCTCTC-3′ Reverse: 5′-TTCGCAGATCCGATTTCTCT-3′ 116 bp
CRLF2 Forward: 5′-TGCACCAACTACCTTCTCCA-3′ Reverse: 5′-TGATGCCACGAAAATCTCAC-3′ 182 bp
IL3RA Forward: 5′-TTAAGCAGGCACCTCTGTCC-3′ Reverse: 5′-CTGAGCCTTTGCTTTCATCC-3′ 154 bp

PCR, polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; CSF2RA, colony stimulating factor 2 receptor, alpha; CRLF2, cytokine receptor-like factor 2; IL3RA, interleukin 3 receptor, alpha.