FIG. 2.

RNA dot blot experiments. Equal volumes and concentrations of total RNA (6 μg) from Rlt100 cultures grown in defined media with either glycerol-glucose or glycerol-rhamnose as carbon sources were spotted onto nylon charged membranes and probed with probes specific for rhaR, rhaS, rhaT, and rhaK as described in Materials and Methods. The figure shows the results of one representative experiment. Ratios represent the quantitated glucose values divided by the quantitated rhamnose values derived from three independent experiments. Values in parentheses represent the standard deviations.