FIG. 3.
Rhamnose transport experiments. Strains were grown to mid-log phase in defined medium containing either glucose-glycerol (open symbols) or rhamnose-glycerol (closed symbols), and initial uptake rates were determined using [3H]rhamnose. Symbols: squares, Rlt100 (wild-type); diamonds, Rlt117 (rhaR25); triangles, Rlt112 (rhaS17); circles, Rlt106; +, Rlt151 (rhaQ38); X, Rlt128 (rhaP36). (A) Comparison of rhamnose uptake by Rlt100 grown on rhamnose-glycerol or glucose-glycerol. (B) Comparison of uptake of induced wild-type and representative mutants. Note that symbols for the mutants are so low that they are superimposed. The data represent the averages of three independent experiments. Experiments in both panels were carried out at the same time. Error bars are smaller than the symbols in all cases.