TABLE 3.
Effect of rhamnose and glucose on β-galactosidase fusions to genes in the rhamnose transport operon
Strain | Relevant genotype | Glucosea | Rhamnose | Inductionb |
---|---|---|---|---|
Rlt117 | rhaR25::Tn5B20 | 2,824 (185)c | 10,451 (751)d | 3.7 |
Rlt128 | rhaP36::Tn5B20 | 24 (1) | 660 (46)e | 28 |
Rlt151 | rhaQ38::Tn5B20 | 33 (4) | 424 (41) | 13 |
Rlt131 | rhaK40::Tn5B20 | 2 (1) | 5 (0) | 2 |
Rlt144 | rhaK50::Tn5B20 | 2 (1) | 11 (2)e | 5 |
Rlt146 | rhaK52::Tn5B20 | 4 (2) | 12 (1)e | 3 |
The values given represent β-galactosidase activity expressed in Miller units following overnight growth in defined media with either glucose-glycerol or rhamnose-glycerol as carbon sources. Values are the average of at least four independent replicates; values in parentheses represent standard error.
Ratio of rhamnose values to the glucose values.
Value represents seven independent replicates.
Value represents six independent replicates.
Value represents three independent replicates.