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Effect of membrane localization on stability and activity of TcpP. (A) Schematic diagram of the TcpP truncates constructed. The truncates were expressed from the inducible plasmid pBAD18. (B) Western blot analysis of the ΔtcpP strain transformed with the truncates illustrated above. (C) β-Galactosidase assay of ΔtcpP strains containing a chromosomal toxT-lacZ reporter fusion transformed with the truncates described above.
