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Effect of tcpH and toxS deletion on TcpP and ToxR, respectively. (A) Western blot analysis of TcpP levels in wild-type (WT), ΔtcpH and ΔtcpH + tcpH(pACYC184) cultures. (B) Western blot analysis of ToxR levels in the wild type (WT), ΔtoxS, and ΔtoxR. For both analyses, cells were grown to mid-log phase in LB medium at pH 6.5 at 30° C.
