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Effect of tcpH deletion on the level of tcpP transcript. RT-PCR analysis of RNA transcript from wild-type (WT), ΔtcpP, ΔtcpH, and ΔtcpH + tcpH (pACYC184) cultures RNA was harvested from cultures grown to mid-log phase in LB medium at pH 6.5 and 30°C. (A) The TcpP-specific product is shown. (B) The RNA polymerase α-subunit (rpoA)-specific product is shown to demonstrate that levels of RNA have been normalized. +RT, with reverse transcriptase; −RT, without reverse transcriptase.
